IL-5, IL-8 and tgf-beta expressions in chronic rhinosinusitis patients with nasal polyps and their correlation to tissue's cellularity and disease relapsing

Methods The mRNA expression of the IL-5, IL-8 and TGF-beta protein was analyzed using qRT-PCR in 32 nasal polyps and 7 control samples comparing it with medium turbinate control samples (obtained from patients undergoing aesthetic rhinoplasty). The numbers of eosinophils and neutrophils were counted in samples obtained during surgical procedure, and stained with hematoxylin eosin in an optical microscope. The patients were followed up to three years after surgery, and considered with relapsing disease if there was polyp in the medium meatus through nasal endoscopy. Results IL-5 and IL-8 expressions were increased whereas TGFbeta was decreased in the nasal polyps when compared to the control mucosa from medium turbinates. There was no correlation between these molecules expression and tissue’s cellularity, nor to relapsing of the disease.